Author

• Negar Nazemi

Tehran university

Abstract

Introduction to Liquid Chromatography Instruments, Spectrophotometer, and ELISA Reader in Toxicology Laboratory
Liquid Chromatography System
The liquid chromatography system, or HPLC, which stands for High Performance Liquid Chromatography, is located in the toxicology laboratory, entomology section, on the third floor. This system is mainly used in this section to measure pesticide residues in agricultural products, soil, and water.
Structurally, this device has detectors. The UV detector identifies compounds that absorb UV light. There is also an RID detector used for measuring sugars, which is currently used less in this laboratory. The system has two pumps responsible for maintaining the flow of the mobile phase. In the device oven, we have different types of columns, mainly C18 columns, which are used for various pesticides. The device has an autosampler used for automatic sampling and measuring of samples. It also has a data recording section where the chromatograms obtained from this system are recorded, printed, and analyzed to measure pesticide residues.

Keywords

• Spectrophotometer'
• ' ELISA reader'
• ' Chromatography

Main Subjects

• گزارش